EVALUATION OF THE ANTIBACTERIAL EFFICACY OF Ocimum gratissimum XTRACT ON Klebsiella SPECIES ISOLATES OF CLINICAL AND FOOD ORIGIN
IMONI, A. A., AGBONWANETEN, O. B. AND OTEGA, E.
ABSTRACT
This study evaluated the antibacterial efficacy of Ocimum gratissimum leaf extracts against Klebsiella species isolates of food and clinical origin. The isolates were identified using cultural, morphological, and biochemical characteristics. Aqueous and ethanolic extracts of Ocimum gratissimum were prepared. Thereafter, extract was screened for the presence of bioactive constituents such as tannin, phenol and phlobatannin. Antibiotic susceptibility test was carried out using the Kirby – Bauer disc diffusion method. Antibacterial activity was determined by agar well diffusion technique, while at concentrations ranging from 125mg/ml to 2000mg/ml, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was also established. Six (6) Klebsiella isolates were….