MOLECULAR DETECTION AND ANTIBIOGRAM CHARACTERIZATION OF Staphylococcus aureus STRAINS ISOLATED FROM URINE SAMPLES IN A TERTIARY HOSPITAL BASED IN BENIN CITY, NIGERIA – (2016) ODJADJARE, E. E. O. AND EKRAKENE, T
ABSTRACT
The prevalence and antibiotic susceptibility profiles of Staphylococcus aureus strains isolated from urine samples at the University of Benin Teaching Hospital (UBTH), Nigeria, was investigated. A total of 101 bacterial isolates from urine samples were collected from the Microbiology laboratory of UBTH and identified using standard microbiological techniques. Isolates identity were further confirmed by polymerase chain reaction (PCR) and subjected to antibiotic susceptibility testing according to the description of Clinical Laboratory Standard Institute (CLSI). Thirty-seven of the 101 bacterial isolates (37%) were presumptively identified as Staphylococcus aureus using cultural/biochemical methods, out of which 16 (43%) were confirmed as strains of Staphylococcus aureus by PCR technique. The PCR confirmed isolates exhibited resistance to ampicillin (100%), ceftazidime (81%), cefuroxime (75%), ceftriaxone (56%) and tetracycline (50%). Multiple antibiotic resistance (MAR) ranging from 4 to 11 antibiotics with MAR indices of 0.21 to 0.57 were observed among the bacterial isolates. This study demonstrated that multidrug-resistant strains of S. aureus were frequently associated with urinary tract infections in Benin City, Nigeria and reaffirms the hospital
setting as an important reservoir for this pathogen. It is therefore incumbent on relevant stakeholders to continually and regularly place this bacterial pathogen under close surveillance with a view to curbing its spread and preserve the public health. Download PDF